Requests for services are made within the UWBC Requester Portal, where you will have access to a guided submission process, be able to monitor the status of all requests on the lab-associated account, and see specific charges attributed to each request.
Before you deliver samples to the DNA Sequencing facility, please look at all applicable guidelines in the expandable menu below.
To access the UWBC Requester Portal, you must first be registered by UWBC staff. To request access, email nextgen-seq@biotech.wisc.edu.
Once registered, you can access the UWBC Requester Portal at any time. All feedback on the Portal is welcome so that we can continue to improve the experience and expand available features within the Portal for all the researchers we serve!
If you are having issues with the UWBC Requester Portal registration or you just need step by step guidance for the process, please check out this walkthrough.
This is an accordion element with a series of buttons that open and close related content panels.
Guidelines for Submitting DNA for Library Preparation or Already Prepared Libraries
The DNA Sequencing Facility provides sequencing of prepared libraries and library preparation for multiple applications and sequencing services. Click the link below to view the guidelines applicable to your service request.
Libraries Already Prepared (Illumina)
GBS or ddRAD Library Preparation (Illumina)
Whole Genome Library Preparation (multiple sequencing platforms)
Amplicon Library Preparation – 16s, 18s, ITS, custom (multiple sequencing platforms)
Index PCR Library Preparation (Illumina)
Other DNA (e.g., cDNA, plasmid, ChIP) Library Preparation (multiple sequencing platforms)
Guidelines for Submitting Biological Samples (e.g., Tissues, Cells, Other Inputs) for DNA Extraction
The DNA Sequencing Facility Provides DNA extraction services for downstream use in multiple applications and on multiple sequencing platforms. The guidelines below are grouped based on application/platform.
Standard DNA Extraction (Illumina – all applications, Oxford Nanopore/PacBio – metagenomics and amplicon)
-
- Plant tissues
- Whole blood
- Animal, insect, other tissues
- Animal and bacterial cells
- Saliva or buccal cells
- Fecal and soil samples
- Environmental (dust, swab, filter, etc.) samples
High Molecular Weight DNA Extraction (Oxford Nanopore/PacBio – whole genome)
-
- Plant tissues
- Whole blood
- Animal tissues
- Animal and bacterial cells
Guidelines for Submitting DNA or Libraries for QC or Other Non-Sequencing Services
The DNA Sequencing Facility provides additional a la carte services. Note that some of these services may be included in library preparation and sequencing services. Click the link below to view the guidelines applicable to your service request.
Standard DNA QC (quantification and size analysis)
High Molecular Weight DNA QC (NanoDrop, quantification, size analysis with Femto Pulse)
DNA Shearing (Covaris M220 focused-ultrasonication)
DNA Size Selection (PippinHT or BluePippin)
PCR and Other DNA Cleanup (SPRI bead Cleanup and Size Selection)
High- and Low-Throughput Fluormetric Quantification
Guidelines for Sample Delivery
The DNA Sequencing Facility has multiple ways for you to deliver your DNA, libraries, or biological samples.
UWBC Freezer – Only DNA or libraries may be submitted in this fashion. No biological samples (tissues, cells, etc.) allowed in this freezer.
By Appointment – Only for submission of biological samples (tissues, cells, etc.). DNA and library submission should be done via the UWBC freezer.
Shipping – For submission of DNA, libraries, or biological samples (tissues, cells, etc.) by courier.