Short Read Sequencing

Service Description

Our Whole genome sequencing (WGS) service provides a broad overview of the entire genome. This type of sequencing only requires 10ng of input DNA and allows researchers to explore the genome for both small and large variants. WGS can be done on a multitude of organisms such as human, bacteria, fungi, and plants as well as metagenomes. Depending on the sequencing required for the project, WGS libraries can be run on either Illumina’s MiSeq or NovaSeq 6000.

Genome Assembly

Whole genome sequencing can be used for reference based sequencing or assembly, the comparison of new sequencing to a known reference sequencing. Conversely, whole genome sequencing is also ideal for discovering novel genomes because it offers a large amount of data to help characterize/identify any species. We recommend combining long read and short read sequencing to create a comprehensive genome assembly. Check out the Whole Genome Sequencing – Long Read page for more information.

Metagenomic Sequencing

Metagenomic sequencing allows researchers to analyze all microbial genomes present within one sample collection. It allows researchers to study microbial genomes that otherwise can’t be cultivated. This method can be performed using DNA from various types of environmental samples such as air, soil, aquatic sediment, microbiome, city (subways), and marine. If you are interested in specifically targeting the bacteria, fungi, or eukaryotic organisms within an environmental sample, then please check out the 16s/18s/ITS Amplicon Sequencing page