Long Read Sequencing

LONG READ SEQUENCING

Service Description

Our long read whole genome sequencing (WGS) service utilizes Oxford Nanopore Technologies and Pacific Biosciences (PacBio) platforms to characterize your novel genome of interest or re-characterize organisms with known genomes. Our team has extensive experience with a variety of sample types – we are continuously attempting new preps and sequencing protocols to push the boundaries of what these platforms can offer.

General Project Progression

De novo genome assembly

Our de novo genome assembly approach uses both ultra-long Oxford Nanopore Technologies reads alongside PacBio HiFi data. The ultra-long Oxford Nanopore Technologies reads are upwards of 100kb and the HiFi reads are 10-20kb in length with 99.8% accuracy; the combination of these two technologies results in extremely powerful data to create high quality genome assemblies. De novo genome assembly projects are tailored to your needs and depend on organism type and genome size; please contact us if you are interested in this service. If you require bioinformatic analysis, our group is highly qualified and experienced with these types of projects – please contact the BRC here.

Recommendations

Long read sequencing, requires extremely clean high molecular weight (HMW) DNA. This can be challenging for some organism types. Please see our HMW DNA Extraction page if this service would benefit your needs.

Access our Interactive Submission Guidelines and get the exact guidance you need to prepare, label, and deliver your samples.

If you can’t find the information you’re looking for, please contact us at nextgen-seq@biotech.wisc.edu

High Molecular Weight DNA QC and Extraction Pricing

Effective 7/8/24

Service	Details	UW System Setup Rate	UW System Per Sample Rate	External Setup Rate	External Per Sample Rate
High Molecular Weight DNA QC	Setup charge for 1-88 concurrent samples + per sample charge	168	31	~$259**	~$48**
High Molecular Weight DNA Extraction from Plant Leaf Tissue*	Setup charge per batch of 16 samples + per sample charge	1050	90	~$1610**	~$138**
High Molecular Weight DNA Extraction from Blood*	Setup charge per batch of 16 samples + per sample charge	490	117	~$751**	~$179**
High Molecular Weight DNA Extraction from Animal Tissue*	Setup charge per batch of 16 samples + per sample charge	710	130	~$1089**	~$199**

* High molecular weight DNA QC is included in the cost of extraction. If your input isn't explicitly listed, please inquire.
** For exact cost, please inquire for a quote.

* High molecular weight DNA QC is included in the cost of extraction. If your input isn't explicitly listed, please inquire.

** For exact cost, please inquire for a quote.

Prices are subject to change without notice. Please email nextgen-seq@biotech.wisc.edu to request a quote. In the absence of a valid quote, prices from our current price books will be applied at the time of the service request. Prices may differ from those listed on this page.

Prices are subject to change without notice. Please email nextgen-seq@biotech.wisc.edu to request a quote. In the absence of a valid quote, prices from our current price books will be applied at the time of the service request. Prices may differ from those listed on this page.

Oxford Nanopore Technologies Service Pricing

Effective 7/8/24

Service	Details	UW System Setup Rate	UW System Per Sample Rate	External Setup Rate	External Per Sample Rate
LSK114 Library Preparation	Setup charge for 1-24 concurrent samples + per sample charge	350	190	~$510**	~$277**
NBD114 Library Preparation	Setup charge for 1-24 concurrent samples + per sample charge	585	195	~$866**	~$289**
RBK114 Library Preparation	Setup charge for 1-96 concurrent samples + per sample charge	360	15	~$569**	~$24**
PromethION Flow Cell (72 hour)	Setup charge for 1-4 flow cells + per flow cell charge; 72 hour run	570	1100	~$645**	~$1245**
PromethION Flow Cell (12 hour)	Per prorated flow cell; 12 hour run		585		$670
Ultra-Long*	Setup charge for 1-3 concurrent samples + per sample charge	1200	2800	~$1387**	~$3236**

* Ultra-long service includes DNA extraction, library preparation, and sequencing on one PromethION flow cell per sample.
** For exact cost, please inquire for a quote.

* Ultra-long service includes DNA extraction, library preparation, and sequencing on one PromethION flow cell per sample.

** For exact cost, please inquire for a quote.

Prices are subject to change without notice. Please email nextgen-seq@biotech.wisc.edu to request a quote. In the absence of a valid quote, prices from our current price books will be applied at the time of the service request. Prices may differ from those listed on this page.

PacBio Service Pricing

Effective 7/8/24

Service	Details	UW System Setup Rate	UW System Per Sample Rate	External Setup Rate	External Per Sample Rate
HiFi Library Preparation	Setup charge for 1-24 concurrent samples + per sample charge	980	255	~$1310**	~$341**
Amplicon Library Preparation	Setup charge for 1-96 concurrent samples + per sample charge	620	100	~$980**	~$158**
Revio SMRT Cell*	Per 25M SMRT Cell		2300		$2475

* Cost of sequencing includes consensus calls from subreads (HiFi reads from Circular Consensus Sequencing).
** For exact cost, please inquire for a quote.

* Cost of sequencing includes consensus calls from subreads (HiFi reads from Circular Consensus Sequencing).

** For exact cost, please inquire for a quote.

Prices are subject to change without notice. Please email nextgen-seq@biotech.wisc.edu to request a quote. In the absence of a valid quote, prices from our current price books will be applied at the time of the service request. Prices may differ from those listed on this page.

Unless otherwise specified, all data and reagents distributed by the University of Wisconsin Biotechnology Center DNA Sequencing Facility are intended for research purposes only. They are not intended nor certified for diagnostic or clinical use. Clinical services are provided through our collaboration with the UW Collaborative Genomics Core.