GBS
Service Description
GBS (Genotype By Sequencing) is a cost-effective, high-throughput service that helps in the discovery of single nucleotide polymorphisms (SNP) for plant and animals along with genotyping studies. If your species does not have a known enzyme combination for GBS, we offer optimization/restriction enzyme selection! We’ve taken the guesswork out of sequencing selection by pairing library preparation and sequencing in our GBS Full-service option.
- We offer single and dual enzyme digest (ApeKI, PstI/MspI, PstI/BfaI, NsiI/MspI, NsiI/BfaI)
- ddRAD and RADseq can be done with the inclusion of a size selection step
- GBS Full-service includes library preparation and ≥250 million reads per plate (2 x 150 bp, NovaSeq X Plus)
- Full lanes of a 2 x 150 bp 10B flow cell can also be cost-effective sequencing options on the NovaSeq X Plus
- Large projects >20 plates: Full 2 x 50 bp or 2 x 100 bp 10B flow cells may provide a cost advantage – please inquire for a quote
- UWBC Bioinformatics Resource Center has vast experience with downstream analysis
- Licensed service provider*
- Sequencing of client-prepared GBS libraries**
*The University of Wisconsin-Madison Biotechnology Center 
– DNA Sequencing Facility is a Licensed Service Provider,
for internal and external clients, providing GBS services under license from Keygene N.V. which owns patents and patent applications protecting its Sequence Based Genotyping technologies.
**KeyGene license fee will be applied to any client-prepared libraries
Optimization of Species
Species’ genomes respond differently to combinations of restriction enzymes, this can all be impacted by the G/C content, methylation status, or complexity amongst other conditions. If the enzyme combination is not known yet, species will need to have optimization/restriction enzyme selection performed to determine which enzymes to proceed with for GBS. We have worked with a variety of different species and are open to working with new species. Please contact us at nextgen-seq@biotech.wisc.edu to discuss your project.
Advantages
We have updated our GBS prep protocol to now include post-PCR Normalization! Post-PCR normalization provides data with a more even distribution of reads across a 96 well plate. This ensures that low concentration samples get even coverage. A comparison of the number of reads per GBS samples with or without post-PCR normalization can be seen in two different plates of samples in Figure 1 and 2.
GBS Data Figures 1 & 2
Access our Interactive Submission Guidelines and get the exact guidance you need to prepare, label, and deliver your samples.
If you can’t find the information you’re looking for, please contact us at nextgen-seq@biotech.wisc.edu
GBS Pricing
Effective 7/8/24
| * Optimization is recommended for species that don't have published GBS data. Up to 4 species can be optimized concurrently at a prorated cost; inquire for quote. |
| ** Prep based on 96-well plate and the following enzymes: ApeKI, PstI/MspI, PstI/BfaI, NsiI/MspI, NsiI/BfaI. Sequencing is a guaranteed 250 million reads per plate. |
| Prices are subject to change without notice. Please email nextgen-seq@biotech.wisc.edu to request a quote. In the absence of a valid quote, prices from our current price books will be applied at the time of the service request. Prices may differ from those listed on this page. |
Unless otherwise specified, all data and reagents distributed by the University of Wisconsin Biotechnology Center DNA Sequencing Facility are intended for research purposes only. They are not intended nor certified for diagnostic or clinical use. Clinical services are provided through our collaboration with the UW Collaborative Genomics Core.