ChIP-Seq

Service Description

ChIP-Seq (chromatin immunoprecipitation sequencing) allows for genome-wide mapping of DNA binding proteins and histone modifications. It is primarily used to determine how proteins influence the mechanisms that cause phenotypic appearance. The advantage of ChIP-Seq over other assays is that ChIP-Seq does not require prior knowledge of the epigenome.

Submission

For this service, researchers are responsible for submitting properly fragmented and immunoprecipitated DNA for library preparation to be completed. ChIP-Seq can be run on our Illumina NovaSeq or MiSeq.

Access our Interactive Submission Guidelines and get the exact guidance you need to prepare, label, and deliver your samples.

If you can’t find the information you’re looking for, please contact us at nextgen-seq@biotech.wisc.edu

Illumina ChIP Library Prep Pricing

Effective 7/8/24

Service	Details	UW System Setup Rate	UW System Per Sample Rate	External Setup Rate	External Per Sample Rate
Illumina ChIP Library Prep	Setup charge for 1-96 concurrent samples + per sample charge	688	135	~$986*	~$194*

* For exact cost, please inquire for a quote.

* For exact cost, please inquire for a quote.

Prices are subject to change without notice. Please email nextgen-seq@biotech.wisc.edu to request a quote. In the absence of a valid quote, prices from our current price books will be applied at the time of the service request. Prices may differ from those listed on this page.

Prices are subject to change without notice. Please email nextgen-seq@biotech.wisc.edu to request a quote. In the absence of a valid quote, prices from our current price books will be applied at the time of the service request. Prices may differ from those listed on this page.

NovaSeq X Plus Pricing

Effective 7/8/24

Service	Details	UW System Rate	External Rate
1.5B 2x50 Full Flowcell	Per Flow Cell (1.5B reads)**	3870	5405
1.5B 2x100 Full Flowcell	Per Flow Cell (1.5B reads)**	4470	6060
1.5B 2x150 Full Flowcell	Per Flow Cell (1.5B reads)**	4710	6335
10B 2x50 Full Flowcell	Per Flow Cell (10B reads)**	10565	12660
10B 2x100 Full Flowcell	Per Flow Cell (10B reads)**	12875	15190
10B 2x150 Shared Lane (10M read increments)	Per 10M reads targeted*	60	66
10B 2x150 Half Lane (450M reads)	Per 450M reads targeted*	895	1160
10B 2x150 10B Full Lane	Per Lane (1.25B reads)**	1790	2320
10B 2x150 10B Full Flowcell	Per Flow Cell (10B reads)**	14320	18055
25B 2x150 Full Flowcell	Per Flow Cell (25B reads)**	22235	26710

* M=million. This is targeted number of reads. Actual read counts may vary ±20% from targeted amounts.
** B=billion. Read specifications based on Illumina PhiX control library. Output may vary based on library type.

* M=million. This is targeted number of reads. Actual read counts may vary ±20% from targeted amounts.

** B=billion. Read specifications based on Illumina PhiX control library. Output may vary based on library type.

Prices are subject to change without notice. Please email nextgen-seq@biotech.wisc.edu to request a quote. In the absence of a valid quote, prices from our current price books will be applied at the time of the service request. Prices may differ from those listed on this page.

Unless otherwise specified, all data and reagents distributed by the University of Wisconsin Biotechnology Center DNA Sequencing Facility are intended for research purposes only. They are not intended nor certified for diagnostic or clinical use. Clinical services are provided through our collaboration with the UW Collaborative Genomics Core.