16S and ITS
Service and Sequencing Platform Change (Effective 3/31)
- Minimum sample threshold lowered: The minimum sample count for this service has been lowered to 12 samples. A minimum sample threshold keeps our service costs down and our turnaround times lower.
- MiSeq i100 Plus: We welcome our newest sequencing platform, the MiSeq i100 Plus, which offers many of the same great qualities that the NextSeq 2000 did.
- Low cost/sample: Costs remain the same as they were with the NextSeq 2000.
- Flexibility: A 5M and 25M flow cell allow us to adapt to small and large projects, thus helping us further reduce turnaround times.
- Quality: The quality of MiSeq i100 reads is just as great as the quality of NextSeq 2000 reads!
18s amplicon prep and sequencing services have been discontinued.
Please feel free to contact us at nextgen-seq@biotech.wisc.edu to discuss alternative options or other resources.
Service Description
We provide cost-effective, high-throughput analysis of microbiomes through a metabarcoding approach. We target the 16S rRNA gene or ITS (Internal Transcribed Spacer) region using primers designed to conserved regions, which amplify across variable regions to determine the various taxa in your microbiomes. Our service provides Illumina library preparation coupled with sequencing on our MiSeq i100 Plus so that you don’t need to worry about read length and depth of sequencing. The depth of sequencing we provide is sufficient for most typical microbiomes (fecal/gut, skin) and even those with high biodiversity such as soil.
16S We amplify a single product containing the V3 and V4 hypervariable regions of the 16S rRNA gene. The primers used are widely published and highly suited for detection of a large spectrum of bacteria at the phylum level.
ITS We amplify a single product containing ITS1 and ITS2. The primers used are ideal for identification of fungi. Data from the ITS region is advantageous for fungal phylogenetic analysis because this region has more variation than the ribosomal sequences (18S).
Alternatives and Complementary Services
Are you interested in the microbiome, but require different primers from those we use? Please check out the index PCR sequencing services pages.
If you aren’t satisfied with what a metabarcoding approach or you need higher taxonomic resolution, consider a metagenomics approach using whole genome metagenomics. It may be more affordable than you think!
To complement your metabarcoding project, we provide DNA extraction and can work with soil, feces, swabs, and many other environmental sample inputs.
Our expert sequencing specialists can help you delve into any microbial diversity research project and find the best fit for you. Please contact nextgen-seq@biotech.wisc.edu with any questions.
Access our Interactive Submission Guidelines and get the exact guidance you need to prepare, label, and deliver your samples.
If you can’t find the information you’re looking for, please contact us at nextgen-seq@biotech.wisc.edu
Amplicon Library Prep and Sequencing Pricing
Effective 3/31/25
| * Includes Illumina library preparation (16s or ITS) and sequencing with 2x300 bp on MiSeq i100. Sequencing provides ~60,000 reads or more per sample. Due to sample quality and quantity variation, there is no minimum read guarantee. |
| ** Price ranges are based on the number of samples submitted. Please inquire for a quote. |
| Prices are subject to change without notice. Please email nextgen-seq@biotech.wisc.edu to request a quote. In the absence of a valid quote, prices from our current price books will be applied at the time of the service request. Prices may differ from those listed on this page. |
Unless otherwise specified, all data and reagents distributed by the University of Wisconsin Biotechnology Center DNA Sequencing Facility are intended for research purposes only. They are not intended nor certified for diagnostic or clinical use. Clinical services are provided through our collaboration with the UW Collaborative Genomics Core.