16S/18S/ITS
Improved Service, Lower Prices!
- Lower cost/sample for all sized projects – as much as $15/sample less for small projects and $11/sample less for large projects
- Increased average read counts and improved quality on the 3′ ends of reads – NextSeq 2000 2 x 300 bp flow cells provide 4-16x more reads of higher quality compared to a MiSeq 2 x 300 bp flow cell
- Reduced wait times for small projects and the same short wait time for large projects
- Less guesswork – one service option for library prep and sequencing without worrying about coverage
Service Description
We provide cost-effective, high-throughput analysis of microbiomes through a metabarcoding approach. We target the 16S rRNA gene, 18S rRNA gene, or ITS (Internal Transcribed Spacer) region using primers designed to conserved regions, which amplify across variable regions to determine the various taxa in your microbiomes. Our service provides Illumina library preparation coupled with sequencing on our NextSeq 2000 so that you don’t need to worry about read length and depth of sequencing. The depth of sequencing we provide is sufficient for most typical microbiomes (fecal/gut, skin) and even those with high biodiversity such as soil.
16S We amplify a single product containing the V3 and V4 hypervariable regions of the 16S rRNA gene. The primers used are widely published and highly suited for detection of a large spectrum of bacteria at the phylum level. For species- and strain-level detection, we offer full-length 16s rRNA amplicon prep and sequencing on our PacBio Sequel II platform – more information about that service can be found here.
18S We amplify across the V9 hypervariable region of the 18S rRNA gene. The primers used are similar to those indicated by Earth Microbiome Project and allow for detection of eukaryotes such as fungi. This design doesn’t exclude mammalian 18s, so caution should be taken if host contamination is a concern.
ITS We amplify a single product containing ITS1 and ITS2. The primers used are ideal for identification of fungi. Data from the ITS region is advantageous for fungal phylogenetic analysis because this region has more variation than the ribosomal sequences (18S).
Alternatives and Complementary Services
Are you interested in the microbiome, but require different primers from those we use? Please check out the index PCR and custom amplicon sequencing services pages.
If you aren’t satisfied with what a metabarcoding approach provides, consider a metagenomics approach using whole genome metagenomics.
To complement your metabarcoding project, we provide DNA extraction and can work with soil, feces, swabs, and many other environmental sample inputs.
Our expert sequencing specialists can help you delve into any microbial diversity research project and find the best fit for you. Please contact nextgen-seq@biotech.wisc.edu with any questions.
Access our Interactive Submission Guidelines and get the exact guidance you need to prepare, label, and deliver your samples.
If you can’t find the information you’re looking for, please contact us at nextgen-seq@biotech.wisc.edu
Amplicon Library Prep and Sequencing Pricing
Effective 6/7/24
| * Includes 16s, 18s, or ITS Illumina library preparation and sequencing with 2 x300 bp on NextSeq 2000. Sequencing provides ~70,000 reads or more per sample. Due to sample quality and quantity variation, there is no minimum read guarantee. |
| ** Price ranges are based on the number of samples submitted. Please inquire for a quote. |
| Prices are subject to change without notice. Please email nextgen-seq@biotech.wisc.edu to request a quote. In the absence of a valid quote, prices from our current price books will be applied at the time of the service request. Prices may differ from those listed on this page. |
Unless otherwise specified, all data and reagents distributed by the University of Wisconsin Biotechnology Center DNA Sequencing Facility are intended for research purposes only. They are not intended nor certified for diagnostic or clinical use. Clinical services are provided through our collaboration with the UW Collaborative Genomics Core.