3730XL GENETIC ANALYZER–SANGER SEQUENCING
The 3730xl Genetic Analyzer (ThermoFisher) is economical and ideal for small projects of a select number of samples needing information on small regions. The charged products labeled with dyes that are excited while migrating across the detection cell of an array can provide the sizing or sequence.
Sanger Sequencing uses a dye terminator reaction. This can be used to sequence PCR products, verification of insertion/deletion, or point mutation,
Fragment Analysis is the sizing of label PCR product with an internal ladder. Common experiments are AFLP, IDAA, RFLP and microsatellites which can be multiplexed of sizes 100-600bp.
To discuss a project or acquire a quote, please contact us! For new clients, please set up an account (see New Lab or Customer Access below). All projects must be discussed before samples are submitted.
If you would like to initiate a project or get more information, please use the following contact information, and a member of our group will get in touch with you directly.
Next Generation Sequencing: firstname.lastname@example.org
New Lab or Customer Access
We MUST have this information before we can proceed with your request.
Adding a new lab:
- Name, address, phone number, UW NetID and email of the Principal Investigator (PI) or Primary scientific contact person.
- Name, address, phone number, FAX number and email of he financial contact person.
- Name, UW NetID, phone number and email of all individuals who will be using our service.
- Payment information: For campus users this should be a direct charge number, e.g. a 144 or 133 prefixed account (please send the whole string of characters). For off campus users you can set up a PO or we can send the financial contact person a bill — your choice. Non-UW system users are charged an additional institutional overhead.
Adding a member to an existing lab:
- Name of the PI whose lab you are joining.
- Your name, UW NetID, phone number and email.
Sanger Sequencing Submission Guidelines
Please contact email@example.com. Samples can also be dropped off in Room 1259.
Submission Forms and Guidelines
Please check the service page guidelines for specific Submission Forms and Guidelines that need to be included with your samples.
Submitting Samples/ Shipping Instructions
After setting up a customer account, filling out submission form, and reviewing submission guidelines, you can now submit or send samples. *We require customers to submit electronic Submission Forms!*
In Person Submission
Please give advance notice of submission date and time so staff can be prepared to receive samples. Schedule a time for drop off with a staff member at firstname.lastname@example.org. Samples can be dropped off in Room 1259.
Please send the tracking information to:
- Sanger Sequencing: email@example.com
Seal tubes and plates with the matching manufacture caps. Pad the samples to protect against the shipping abuse. Shipping on dry ice is ideal, but for most room temperature is okay. Next Morning delivery is not necessary.
If shipping samples from outside UWBC, ship via express shipping carrier on dry ice to the address below:
UW DNA Sequencing Facility
Attn: Sanger Sequencing
425 Henry Mall
Madison WI, 53706
Pricing for UWBC to run big dye reaction
Customer run big dye reaction and samples cleaned up
Customer run big dye reaction, UWBC provides clean up
Plate is ready for sequencer injection
EXTERNAL RATES: Clients not affiliated with UW Madison or the University of Wisconsin system are charged a 55% surcharge for all labor and incidentals provided; prices are as listed above. This surcharge is subject to change.
Fragment Analysis Pricing
Unless otherwise specified, all data and reagents distributed by the University of Wisconsin Biotechnology Center DNA Sequencing Facility are intended for research purposes only. They are not intended nor certified for diagnostic or clinical use. Clinical services are provided through our collaboration with the UW Collaborative Genomics Core.