SANGER SEQUENCING

SANGER SEQUENCING

Service Description

We offer a variety of cost-effective, high-throughput Sanger Sequencing options. This is still the gold standard for DNA Sequencing.

Data Access

  1. UWBC facilities file access
  2. Globus

Quick Sequencing

The most economical service involves setting up the reaction in your own lab, then submitting the amplified products to our facility. We can go over protocols with you and provide the sequencing reagent, at a discounted rate, to carry out the reactions in your laboratory (a PCR machine is the only required equipment). If you complete the reactions in your lab, but you would like the unincorporated dyes removed, we can purify the products using SPRI beads.

Full Service

We also offer expert service in sequencing if you wish to provide the template DNA and primer. Every effort is made to provide at least 600 bp of excellent sequence (Q score >20), though template composition and purity are crucial determinants of quality. Strategies are available to sequence difficult GC rich templates or homopolymer regions; please indicate if such regions are present.

Plate Options

Different options are available for the processing of 96 well V-bottom plates for either Quick Service or Full Service. Please consult the price list to see what we can do for you if samples are submitted in plates.

Custom

We can also carry out larger sequencing projects involving editing and assembly. Price is based on the degree of confidence required (i.e., single strand vs. double strand, one vs. multiple passes); contact us for more information, dnaseq@biotech.wisc.edu.


Getting Started

To discuss a project or acquire a quote, please contact us! For new clients, please set up an account (see New Lab or Customer Access below). All projects must be discussed before samples are submitted.

If you would like to initiate a project or get more information, please use the following contact information, and a member of our group will get in touch with you directly.

Sanger Sequencing: dnaseq@biotech.wisc.edu


New Lab or Customer Access

We MUST have this information before we can proceed with your request.

Adding a new lab:

    • Name, address, phone number, UW NetID and email of the Principal Investigator (PI) or Primary scientific contact person.
    • Name, address, phone number, FAX number and email of he financial contact person.
    • Name, UW NetID, phone number and email of all individuals who will be using our service.
    • Payment information: For campus users this should be a direct charge number, e.g. a 144 or 133 prefixed account (please send the whole string of characters). For off campus users you can set up a PO or we can send the financial contact person a bill — your choice. Non-UW system users are charged an additional institutional overhead.

Adding a member to an existing lab:

    • Name of the PI whose lab you are joining.
    • Your name, UW NetID, phone number and email.

Sanger Sequencing Submission Guidelines

Please contact dnaseq@biotech.wisc.edu. Samples can also be dropped off in Room 1259.

Submission Forms and Guidelines

Please check the service page guidelines for specific Submission Forms and Guidelines that need to be included with your samples.

Submitting Samples/ Shipping Instructions

After setting up a customer account, filling out submission form, and reviewing submission guidelines, you can now submit or send samples.

In Person Submission

Samples can be dropped off in Room 1259. Or if you want to drop off in person, please email to schedule a time with a staff member at dnaseq@biotech.wisc.edu.

Shipping Submission

Please send the tracking information to:

Seal tubes and plates with the matching manufacture caps. Pad the samples to protect against the shipping abuse. Shipping on dry ice is ideal, but for most room temperature is okay. Next Morning delivery is not necessary.

Sanger Sequencing

UW DNA Sequencing Facility

Attn: Sanger Sequencing

425 Henry Mall

1240 Biotechnology

Madison WI, 53706

Pricing for UWBC to run big dye reaction


Customer run big dye reaction and samples cleaned up


Customer run big dye reaction, UWBC provides clean up


Plate is ready for sequencer injection

EXTERNAL RATES: Clients not affiliated with UW Madison or the University of Wisconsin system are charged a 55% surcharge for all labor and incidentals provided; prices are as listed above.  This surcharge is subject to change.

Unless otherwise specified, all data and reagents distributed by the University of Wisconsin Biotechnology Center DNA Sequencing Facility are intended for research purposes only. They are not intended nor certified for diagnostic or clinical use. Clinical services are provided through our collaboration with the UW Collaborative Genomics Core.