PacBio

PACBIO LIBRARIES

Service Description

The PacBio Sequel II is a powerful long read sequencer that can provide high quality, long read data to achieve a wide variety of research goals. We offer many different PacBio library preps to meet your specific needs. Our long-read sequencing projects are tailored to meet your needs – please contact us about your project and research goals so we can offer consultation and more specific advice depending on your project.

Recommendations

To complement our PacBio services, we offer Oxford Nanopore Technologies library prep and sequencing. We have had great success with projects that use this service performed in tandem with PacBio to fully elucidate long stretches of genomes.

We offer high molecular weight (HMW) extraction if you need assistance in obtaining high quality HMW DNA. Clean, high quality, HMW DNA is essential to the success of library prep and sequencing on the PacBio system.  The mass of DNA required depends on the type of prep you would like us to perform, which can be a large amount in some instances.


Getting Started

To discuss a project or acquire a quote, please contact us! For new clients, please set up an account (see New Lab or Customer Access below). All projects must be discussed before samples are submitted.

If you would like to initiate a project or get more information, please use the following contact information, and a member of our group will get in touch with you directly.

Next Generation Sequencing: nextgen-seq@biotech.wisc.edu


New Lab or Customer Access

We MUST have this information before we can proceed with your request.

Adding a new lab:

    • Name, address, phone number, UW NetID and email of the Principal Investigator (PI) or Primary scientific contact person.
    • Name, address, phone number, FAX number and email of he financial contact person.
    • Name, UW NetID, phone number and email of all individuals who will be using our service.
    • Payment information: For campus users this should be a direct charge number, e.g. a 144 or 133 prefixed account (please send the whole string of characters). For off campus users you can set up a PO or we can send the financial contact person a bill — your choice. Non-UW system users are charged an additional institutional overhead.

Adding a member to an existing lab:

    • Name of the PI whose lab you are joining.
    • Your name, UW NetID, phone number and email.

Sample Submission

Please contact nextgen-seq@biotech.wisc.edu to arrange all sample submissions. Samples and submission forms must be submitted before a project can be entered into our queue.

Submission Forms and Guidelines

Please check the service page guidelines for specific Submission Forms and Guidelines that need to be included with your samples.

Submitting Samples/ Shipping Instructions

After setting up a customer account, filling out submission form, and reviewing submission guidelines, you can now submit or send samples. *We require customers to submit electronic Submission Forms!*

In Person Submission

Please give advance notice of submission date and time so staff can be prepared to receive samples. Schedule a time for drop off with a staff member at nextgen-seq@biotech.wisc.edu

Shipping Submission

Please send the tracking information to:

Next Generation Sequencing: nextgen-seq@biotech.wisc.edu

*Samples that are being shipped must have a hard copy of the submission form and guidelines in the package! This way we know what the samples are and where they are from.*

If shipping samples from outside UWBC, ship via express shipping carrier on dry ice to the address below:

Next Generation Sequencing
UW DNA Sequencing Facility
Attn: Next Generation Sequencing
425 Henry Mall
1250 Biotechnology
Madison WI, 53706

PacBio Sequencing Guidelines

PacBio Submission Form (required with every submission) *Please note: Certain elements are not viewable on Mac Preview!

PacBio Pricing

EXTERNAL RATES: Clients not affiliated with UW Madison or the University of Wisconsin system are charged a 55% surcharge for all labor and incidentals provided; prices are as listed above. This surcharge is subject to change.

Unless otherwise specified, all data and reagents distributed by the University of Wisconsin Biotechnology Center DNA Sequencing Facility are intended for research purposes only. They are not intended nor certified for diagnostic or clinical use. Clinical services are provided through our collaboration with the UW Collaborative Genomics Core.