Oxford Nanopore Technologies

Service Description

As a certified service provider of library preparation and sequencing on the Oxford Nanopore Technologies PromethION 24, we provide high quality, long and ultra-long read data from various DNA and RNA inputs. The PromethION 24 is ideal for large projects, such as complete plant or human genomes while easily scaling to projects with various sample counts and genome sizes. We can run 24 flow cells simultaneously, generating 100Gb for each. The range of possibilities for projects on Oxford Nanopore Technologies is continually expanding at a very exciting rate and we always strive to offer the best service for research needs.

Advantages

Oxford Nanopore Technologies library prep kits allow us to sequence native DNA, eliminating PCR bias from the data. Native DNA in sequencing maintains strand methylation status and allows us to detect DNA base modifications without the need for chemical conversion. The pore-based flow cells allow for superior reading through long repeat regions compared to traditional next-generation sequencing. These long reads are essential for detecting structural variants and completing genome assemblies. Past projects have consistently generated read lengths upwards of 100kb (kilobases). As the only platform that can directly sequence RNA, there is an opportunity to explore splice variants/isoforms as well as base modifications.

Recommendations

To take best advantage of this platform, we recommend that you submit very high-quality DNA. We offer High Molecular Weight Extraction services. We have successfully extracted high molecular and ultra-high molecular weight DNA from a wide variety of microorganisms, plants, and animals. Contact us to go over your project plan so we can best assist with offering Oxford Nanopore Technologies services to meet your goals.

To complement our Oxford Nanopore Technologies services, we offer PacBio HiFi and we have had great success with projects that use this service performed in tandem with Oxford Nanopore Technologies to fully elucidate long stretches of genomes.

The Bioinformatics Resource Center is highly skilled at handling Oxford Nanopore Technologies data. We highly recommend contacting their experts to set up a meeting and work on your project plan.

Access our Interactive Submission Guidelines and get the exact guidance you need to prepare, label, and deliver your samples.

If you can’t find the information you’re looking for, please contact us at nextgen-seq@biotech.wisc.edu

Oxford Nanopore Technologies Service Pricing

Effective 7/8/24

Service	Details	UW System Setup Rate	UW System Per Sample Rate	External Setup Rate	External Per Sample Rate
LSK114 Library Preparation	Setup charge for 1-24 concurrent samples + per sample charge	350	190	~$510**	~$277**
NBD114 Library Preparation	Setup charge for 1-24 concurrent samples + per sample charge	585	195	~$866**	~$289**
RBK114 Library Preparation	Setup charge for 1-96 concurrent samples + per sample charge	360	15	~$569**	~$24**
PromethION Flow Cell (72 hour)	Setup charge for 1-4 flow cells + per flow cell charge; 72 hour run	570	1100	~$645**	~$1245**
PromethION Flow Cell (12 hour)	Per prorated flow cell; 12 hour run		585		$670
Ultra-Long*	Setup charge for 1-3 concurrent samples + per sample charge	1200	2800	~$1387**	~$3236**

* Ultra-long service includes DNA extraction, library preparation, and sequencing on one PromethION flow cell per sample.
** For exact cost, please inquire for a quote.

* Ultra-long service includes DNA extraction, library preparation, and sequencing on one PromethION flow cell per sample.

** For exact cost, please inquire for a quote.

Prices are subject to change without notice. Please email nextgen-seq@biotech.wisc.edu to request a quote. In the absence of a valid quote, prices from our current price books will be applied at the time of the service request. Prices may differ from those listed on this page.

Prices are subject to change without notice. Please email nextgen-seq@biotech.wisc.edu to request a quote. In the absence of a valid quote, prices from our current price books will be applied at the time of the service request. Prices may differ from those listed on this page.

High Molecular Weight DNA QC and Extraction Pricing

Effective 7/8/24

Service	Details	UW System Setup Rate	UW System Per Sample Rate	External Setup Rate	External Per Sample Rate
High Molecular Weight DNA QC	Setup charge for 1-88 concurrent samples + per sample charge	168	31	~$259**	~$48**
High Molecular Weight DNA Extraction from Plant Leaf Tissue*	Setup charge per batch of 16 samples + per sample charge	1050	90	~$1610**	~$138**
High Molecular Weight DNA Extraction from Blood*	Setup charge per batch of 16 samples + per sample charge	490	117	~$751**	~$179**
High Molecular Weight DNA Extraction from Animal Tissue*	Setup charge per batch of 16 samples + per sample charge	710	130	~$1089**	~$199**

* High molecular weight DNA QC is included in the cost of extraction. If your input isn't explicitly listed, please inquire.
** For exact cost, please inquire for a quote.

* High molecular weight DNA QC is included in the cost of extraction. If your input isn't explicitly listed, please inquire.

** For exact cost, please inquire for a quote.

Prices are subject to change without notice. Please email nextgen-seq@biotech.wisc.edu to request a quote. In the absence of a valid quote, prices from our current price books will be applied at the time of the service request. Prices may differ from those listed on this page.

Unless otherwise specified, all data and reagents distributed by the University of Wisconsin Biotechnology Center DNA Sequencing Facility are intended for research purposes only. They are not intended nor certified for diagnostic or clinical use. Clinical services are provided through our collaboration with the UW Collaborative Genomics Core.