Oxford Nanopore Technologies

Platform Description

Oxford Nanopore Technologies offers long-read capabilities on several third-generation sequencing platforms. These platforms have the capabilities to sequence Native and Cycled DNA, cDNA, and RNA through an engineered pore.  The range of possibilities for projects on Oxford Nanopore Technologies is continually expanding at a very exciting rate and we always strive to offer the best service for research needs. We highly recommend meeting to go over your project plan so we can best assist with offering Oxford Nanopore Technologies services and create a Project Summary Plan to meet your goals.

We are Certified Service Providers and run two distinct platforms to optimally fulfill your sequencing depth and coverage needs.


GridION Mk1

We use the GridION for our small to mid-sized projects. We have the flexibility to run up to 5 flow cells at a time, allowing us to work through several projects at a single time or dedicate all the space to a single sample set. We have projects generating upwards of 10Gb per flow cell and this allows us to secure the desired depth of coverage for your DNA project.


PromethION 24

The PromethION 24 allows us to significantly increase the coverage of sequencing projects. We can run 24 flow cells at a time, generating 100Gb for each. This platform is ideal for large projects, such as complete plant or human genomes.


Flongle

A Flongle is a smaller sized flow cell that can be run on the GridION at a lower cost. It offers us the unique opportunity to test parameters prior to fully implementing large projects. It is also ideal for pilot projects and projects that may not require much data.


Data Analysis

The Bioinformatics Resource Center is highly skilled at handling Oxford Nanopore Technologies data. We highly recommend contacting their experts to set up a meeting and work on your project plan.


Getting Started

To discuss a project or acquire a quote, please contact us! For new clients, please set up an account (see New Lab or Customer Access below). All projects must be discussed before samples are submitted.

If you would like to initiate a project or get more information, please use the following contact information, and a member of our group will get in touch with you directly.

Next Generation Sequencing: nextgen-seq@biotech.wisc.edu


New Lab or Customer Access

We MUST have this information before we can proceed with your request.

Adding a new lab:

    • Name, address, phone number, UW NetID and email of the Principal Investigator (PI) or Primary scientific contact person.
    • Name, address, phone number, FAX number and email of he financial contact person.
    • Name, UW NetID, phone number and email of all individuals who will be using our service.
    • Payment information: For campus users this should be a direct charge number, e.g. a 144 or 133 prefixed account (please send the whole string of characters). For off campus users you can set up a PO or we can send the financial contact person a bill — your choice. Non-UW system users are charged an additional institutional overhead.

Adding a member to an existing lab:

    • Name of the PI whose lab you are joining.
    • Your name, UW NetID, phone number and email.

Sample Submission

Please contact nextgen-seq@biotech.wisc.edu to arrange all sample submissions. Samples and submission forms must be submitted before a project can be entered into our queue.

Submission Forms and Guidelines

Please check the service page guidelines for specific Submission Forms and Guidelines that need to be included with your samples.

Submitting Samples/ Shipping Instructions

After setting up a customer account, filling out submission form, and reviewing submission guidelines, you can now submit or send samples. *We require customers to submit electronic Submission Forms!*

In Person Submission

Please give advance notice of submission date and time so staff can be prepared to receive samples. Schedule a time for drop off with a staff member at nextgen-seq@biotech.wisc.edu

Shipping Submission

Please send the tracking information to:

Next Generation Sequencing: nextgen-seq@biotech.wisc.edu

*Samples that are being shipped must have a hard copy of the submission form and guidelines in the package! This way we know what the samples are and where they are from.*

If shipping samples from outside UWBC, ship via express shipping carrier on dry ice to the address below:

Next Generation Sequencing
UW DNA Sequencing Facility
Attn: Next Generation Sequencing
425 Henry Mall
1250 Biotechnology
Madison WI, 53706

Library Preparation Pricing

EXTERNAL RATES: Clients not affiliated with UW Madison or the University of Wisconsin system are charged a 55% surcharge for all labor and incidentals provided; prices are as listed above. This surcharge is subject to change.

Unless otherwise specified, all data and reagents distributed by the University of Wisconsin Biotechnology Center DNA Sequencing Facility are intended for research purposes only. They are not intended nor certified for diagnostic or clinical use. Clinical services are provided through our collaboration with the UW Collaborative Genomics Core.