We offer sequencing services on Illumina Miseq and NovaSeq X Plus instruments. See below how the various run configurations and read lengths we offer can meet your throughput needs!
Illumina MiSeq
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- Single- and paired-end sequencing on a single lane
- Run times vary from 6 hours to 3 days
- Supports longer 300 base pair read lengths
Illumina NovaSeq X Plus
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- High-throughput
- Cost-effective
- Includes service options that don’t require large scale to obtain lower costs
Getting Started
To discuss a project or acquire a quote, please contact us! For new clients, please set up an account (see New Lab or Customer Access below). All projects must be discussed before samples are submitted.
If you would like to initiate a project or get more information, please use the following contact information, and a member of our group will get in touch with you directly.
Next Generation Sequencing: nextgen-seq@biotech.wisc.edu
New Lab or Customer Access
We MUST have this information before we can proceed with your request.
Adding a new lab:
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- Name, address, phone number, UW NetID and email of the Principal Investigator (PI) or Primary scientific contact person.
- Name, address, phone number, FAX number and email of he financial contact person.
- Name, UW NetID, phone number and email of all individuals who will be using our service.
- Payment information: For campus users this should be a direct charge number, e.g. a 144 or 133 prefixed account (please send the whole string of characters). For off campus users you can set up a PO or we can send the financial contact person a bill — your choice. Non-UW system users are charged an additional institutional overhead.
Adding a member to an existing lab:
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- Name of the PI whose lab you are joining.
- Your name, UW NetID, phone number and email.
Sample Submission
Please contact nextgen-seq@biotech.wisc.edu to arrange all sample submissions. Samples and submission forms must be submitted before a project can be entered into our queue.
Submission Forms and Guidelines
Please check the service page guidelines for specific Submission Forms and Guidelines that need to be included with your samples.
Submitting Samples/ Shipping Instructions
After setting up a customer account, filling out submission form, and reviewing submission guidelines, you can now submit or send samples. *We require customers to submit electronic Submission Forms!*
In Person Submission
Please give advance notice of submission date and time so staff can be prepared to receive samples. Schedule a time for drop off with a staff member at nextgen-seq@biotech.wisc.edu
Shipping Submission
Please send the tracking information to:
Next Generation Sequencing: nextgen-seq@biotech.wisc.edu
*Samples that are being shipped must have a hard copy of the submission form and guidelines in the package! This way we know what the samples are and where they are from.*
If shipping samples from outside UWBC, ship via express shipping carrier on dry ice to the address below:
Next Generation Sequencing |
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UW DNA Sequencing Facility Attn: Next Generation Sequencing 425 Henry Mall 1250 Biotechnology Madison WI, 53706 |
- Questions?
Contact us at nextgen-seq@biotech.wisc.edu with any questions.
- Sample Storage
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- All input DNA submitted after 9/12/19, along with any intermediate and/or derived products (to include undiluted stock libraries), will be stored for 6 months and then disposed of.
- All customer prepared libraries submitted after 4/23/18 will be stored for 6 months and then disposed of.
MiSeq Pricing
Effective 3/1/23
* Read specifications based on Illumina PhiX control library. Output may vary based on library type. |
** 2x300 Shared provides ~20,000-70,000 reads per sample. Due to sample quality and quantity variation, there is no minimum read guarantee. |
NovaSeq X Plus Pricing
Effective 8/1/23
* M=million. This is targeted number of reads. Actual read counts may vary ±20% from targeted amounts. |
** B=billion. Read specifications based on Illumina PhiX control library. Output may vary based on library type. |
Illumina pricing is broken into two components: library preparation and run cost. Library preparation is charged on a per sample basis and varies by type. Run charges are based on the length of the sequencing run and whether it is single or paired end. Prices are subject to change due to increasing reagent costs.
Unless otherwise specified, all data and reagents distributed by the University of Wisconsin Biotechnology Center DNA Sequencing Facility are intended for research purposes only. They are not intended nor certified for diagnostic or clinical use. Clinical services are provided through our collaboration with the UW Collaborative Genomics Core.