High Molecular Weight DNA Extraction
Service Description
We provide high molecular weight (HMW) DNA extraction services for whole blood, bacteria, fungi, plant tissue, and mammalian tissue. We follow a variety of published and vetted extraction protocols dependent on the specific sample type. Not all sample types behave the same when following our tissue specific protocols and a few extraction attempts may be necessary before successfully yielding high quality, HMW DNA. We highly recommend that you schedule a meeting with our expert extraction specialists to discuss your project and specific sample type before submission.
Due to the stringency of our downstream sequencing platforms, we perform the following checks to ensure the DNA is high quality prior to working with it.
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- Spectrophotometry with the NanoDrop 1000 – We look for 260/280 ratios at ~1.8 and 260/230 ratios at ~2.0-2.2 to consider the DNA “pure” before continuing.
- Quantification with the Quantus™ Fluorometer – We check that samples have a sufficient DNA concentration to support the next steps of the project.
- Sizing with the Femto Pulse – We look for samples to be >50kb to be considered high quality, HMW DNA.
Sequencing Recommendations
We recommend that HMW DNA is sequenced on our long-read technology that is designed specifically to work with high quality, HMW input. Learn more about Oxford Nanopore Technologies and PacBio.
You can also choose to sequence a portion of your extracted samples on short read technology. If you think this could be beneficial for your project, please check out our WGS- Short Read page for more information.
Please note: All human samples collected during and after November 2019 are considered COVID-19 pandemic specimens. If you would like DNA extracted from these types of samples please contact the facility to discuss the details of your project.
Getting Started
To discuss a project or acquire a quote, please contact us! For new clients, please set up an account (see New Lab or Customer Access below). All projects must be discussed before samples are submitted.
If you would like to initiate a project or get more information, please use the following contact information, and a member of our group will get in touch with you directly.
Next Generation Sequencing: nextgen-seq@biotech.wisc.edu
New Lab or Customer Access
We MUST have this information before we can proceed with your request.
Adding a new lab:
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- Name, address, phone number, UW NetID and email of the Principal Investigator (PI) or Primary scientific contact person.
- Name, address, phone number, FAX number and email of he financial contact person.
- Name, UW NetID, phone number and email of all individuals who will be using our service.
- Payment information: For campus users this should be a direct charge number, e.g. a 144 or 133 prefixed account (please send the whole string of characters). For off campus users you can set up a PO or we can send the financial contact person a bill — your choice. Non-UW system users are charged an additional institutional overhead.
Adding a member to an existing lab:
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- Name of the PI whose lab you are joining.
- Your name, UW NetID, phone number and email.
Sample Submission
Please contact nextgen-seq@biotech.wisc.edu to arrange all sample submissions. Samples and submission forms must be submitted before a project can be entered into our queue.
Submission Forms and Guidelines
Please check the service page guidelines for specific Submission Forms and Guidelines that need to be included with your samples.
Submitting Samples/ Shipping Instructions
After setting up a customer account, filling out submission form, and reviewing submission guidelines, you can now submit or send samples. *We require customers to submit electronic Submission Forms!*
In Person Submission
Please give advance notice of submission date and time so staff can be prepared to receive samples. Schedule a time for drop off with a staff member at nextgen-seq@biotech.wisc.edu
Shipping Submission
Please send the tracking information to:
Next Generation Sequencing: nextgen-seq@biotech.wisc.edu
*Samples that are being shipped must have a hard copy of the submission form and guidelines in the package! This way we know what the samples are and where they are from.*
If shipping samples from outside UWBC, ship via express shipping carrier on dry ice to the address below:
| Next Generation Sequencing |
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| UW DNA Sequencing Facility Attn: Next Generation Sequencing 425 Henry Mall 1250 Biotechnology Madison WI, 53706 |
High Molecular Weight DNA Extraction Guidelines
Submission Forms and Guidelines
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- DNA Extraction Submission Form (required with every submission)
- Guidelines and Sample Sheet (required with every submission)
Submitting Samples
Please provide the guidelines and sample sheet form with sample names, status of the material (live, fixed, etc), biosafety level, and any other relevant safety information. Sample submission guidelines vary depending on the tissue type. Please read through and adhere to the specified instructions for your tissue type of interest.
Sample Types
EXTERNAL RATES: Clients not affiliated with UW Madison or the University of Wisconsin system are charged a 55% surcharge for all labor and incidentals provided; prices are as listed above. This surcharge is subject to change.
Unless otherwise specified, all data and reagents distributed by the University of Wisconsin Biotechnology Center DNA Sequencing Facility are intended for research purposes only. They are not intended nor certified for diagnostic or clinical use. Clinical services are provided through our collaboration with the UW Collaborative Genomics Core.