GBS

GBS

Service Description 

GBS (Genotype By Sequencing) is a cost effective, high throughput service that helps in the discovery of single nucleotide polymorphisms (SNP) for plant and animals along with genotyping studies. If your species does not have a known enzyme combination for GBS, we offer optimization/restriction enzyme selection! We have also improved our GBS prep by adding a post-PCR normalization step.

  • We offer single and dual enzyme digest (ApeKI, PstI/MspI, PstI/BfaI, NsiI/MspI, NsiI/BfaI)
  • ddRAD and RADseq can be done with the inclusion of a size selection step
  • Cost-effective sequencing options on our NovaSeq X+
  • UWBC Bioinformatics Resource Center has vast experience with downstream analysis
  • Licensed service provider*
  • Sequencing of customer prepared GBS libraries

*The University of Wisconsin-Madison Biotechnology Center
– DNA Sequencing Facility is a Licensed Service Provider,
for internal and external clients, providing GBS services under license from Keygene N.V. which owns patents and patent applications protecting its Sequence Based Genotyping technologies.

**KeyGene license fee will be applied to any client-prepared libraries


Optimization of Species 

Species’ genomes respond differently to combinations of restriction enzymes, this can all be impacted by the G/C content, methylation status, or complexity amongst other conditions. If the enzyme combination is not known yet, species will need to have optimization/restriction enzyme selection performed to determine which enzymes to proceed with for GBS. We have worked with a variety of different species and are open to working with new species. Please contact us at nextgen-seq@biotech.wisc.edu to discuss your project.

Advantages 

We have updated our GBS prep protocol to now include post-PCR Normalization! Post-PCR normalization provides data with a more even distribution of reads across a 96 well plate. This ensures that low concentration samples get even coverage. A comparison of the number of reads per GBS samples with or without post-PCR normalization can be seen in two different plates of samples in Figure 1 and 2.

GBS Data Figures 1 & 2

Access our Interactive Submission Guidelines and get the exact guidance you need to prepare, label, and deliver your samples.

 

If you can’t find the information you’re looking for, please contact us at nextgen-seq@biotech.wisc.edu

GBS Pricing

Effective 8/1/23

* Optimization is recommended for species that don't have published GBS data.
** Prep based on 96-well plate and the following enzymes: ApeKI, PstI/MspI, PstI/BfaI, NsiI/MspI, NsiI/BfaI. Sequencing is a guaranteed 250 million reads per plate.


Prices are subject to change due to increasing reagent costs.

Unless otherwise specified, all data and reagents distributed by the University of Wisconsin Biotechnology Center DNA Sequencing Facility are intended for research purposes only. They are not intended nor certified for diagnostic or clinical use. Clinical services are provided through our collaboration with the UW Collaborative Genomics Core.