Exome Capture

Exome Capture

Service Description

Exome sequencing allows researchers to capture the exons, also known as the coding regions, within the genome. This type of library preparation is possible with various types of samples including human, non-human, and formalin-fixed paraffin embedded (FFPE) DNA.

Advantages

By pooling pre-capture libraries prior to capture, we reduce the total number of captures performed, which decreases costs significantly. We have performed an evaluation of this method, pooling 12 samples per capture, and found all samples to have acceptable data quality. The number of pre capture libraries pooled is flexible depending on your project goals and coverage requirements. Pooling pre-capture libraries is what we currently offer as our standard exome service; though, we are happy to accommodate the needs of your specific project.  Please inquire for more information about our exome services.

Exome Sequencing vs. Whole Genome Sequencing

While exome sequencing is only sequencing the exons, whole genome sequencing (WGS) includes everything in the genome: introns (non-coding), exons (coding), and mitochondrial DNA. WGS can result in a lot of data to sort through and may include irrelevant information. Exome sequencing allows researchers to narrow in on a subset or specific gene regions within the exome.

Getting Started

To discuss a project or acquire a quote, please contact us! For new clients, please set up an account (see New Lab or Customer Access below). All projects must be discussed before samples are submitted.

If you would like to initiate a project or get more information, please use the following contact information, and a member of our group will get in touch with you directly.

Next Generation Sequencing: nextgen-seq@biotech.wisc.edu


New Lab or Customer Access

We MUST have this information before we can proceed with your request.

Adding a new lab:

    • Name, address, phone number, UW NetID and email of the Principal Investigator (PI) or Primary scientific contact person.
    • Name, address, phone number, FAX number and email of he financial contact person.
    • Name, UW NetID, phone number and email of all individuals who will be using our service.
    • Payment information: For campus users this should be a direct charge number, e.g. a 144 or 133 prefixed account (please send the whole string of characters). For off campus users you can set up a PO or we can send the financial contact person a bill — your choice. Non-UW system users are charged an additional institutional overhead.

Adding a member to an existing lab:

    • Name of the PI whose lab you are joining.
    • Your name, UW NetID, phone number and email.

Sample Submission

Please contact nextgen-seq@biotech.wisc.edu to arrange all sample submissions. Samples and submission forms must be submitted before a project can be entered into our queue.

Submission Forms and Guidelines

Please check the service page guidelines for specific Submission Forms and Guidelines that need to be included with your samples.

Submitting Samples/ Shipping Instructions

After setting up a customer account, filling out submission form, and reviewing submission guidelines, you can now submit or send samples. *We require customers to submit electronic Submission Forms!*

In Person Submission

Please give advance notice of submission date and time so staff can be prepared to receive samples. Schedule a time for drop off with a staff member at nextgen-seq@biotech.wisc.edu

Shipping Submission

Please send the tracking information to:

Next Generation Sequencing: nextgen-seq@biotech.wisc.edu

*Samples that are being shipped must have a hard copy of the submission form and guidelines in the package! This way we know what the samples are and where they are from.*

If shipping samples from outside UWBC, ship via express shipping carrier on dry ice to the address below:

Next Generation Sequencing
UW DNA Sequencing Facility
Attn: Next Generation Sequencing
425 Henry Mall
1250 Biotechnology
Madison WI, 53706

Please contact us at nextgen-seq@biotech.wisc.edu for exome capture pricing!

Unless otherwise specified, all data and reagents distributed by the University of Wisconsin Biotechnology Center DNA Sequencing Facility are intended for research purposes only. They are not intended nor certified for diagnostic or clinical use. Clinical services are provided through our collaboration with the UW Collaborative Genomics Core.