Automated and Manual DNA Extraction

Automated and Manual DNA Extraction

Service Description

Our team has years of experience handling DNA extractions from a wide variety of samples types from humans, plants, and animals. Some of our favorite projects include animal saliva and bird feathers – just to name a few.  Below you’ll find our most common extraction methods.  If you don’t see your sample type listed, please contact us so we can learn more about your project and see where it fits into our extraction workflow*. We have extensive experience troubleshooting difficult to extract sample types and are excited to tackle your species of interest.

QIACube HT Extractions

© QIAGEN
We utilize the QIACube HT, Qiagen’s automated extraction robot, to perform high throughput extractions in a 96 well plate format for the sample types below.

*Some sample types are in development for the QCHT–such as saliva, but can be extracted with other methods until we fully offer the high-throughput service.

Please note: All human samples collected during and after November 2019 are considered COVID-19 pandemic specimens. If you would like DNA extracted from these types of samples please contact the facility to discuss the details of your project. 

Getting Started

To discuss a project or acquire a quote, please contact us! For new clients, please set up an account (see New Lab or Customer Access below). All projects must be discussed before samples are submitted.

If you would like to initiate a project or get more information, please use the following contact information, and a member of our group will get in touch with you directly.

Next Generation Sequencing: nextgen-seq@biotech.wisc.edu


New Lab or Customer Access

We MUST have this information before we can proceed with your request.

Adding a new lab:

    • Name, address, phone number, UW NetID and email of the Principal Investigator (PI) or Primary scientific contact person.
    • Name, address, phone number, FAX number and email of he financial contact person.
    • Name, UW NetID, phone number and email of all individuals who will be using our service.
    • Payment information: For campus users this should be a direct charge number, e.g. a 144 or 133 prefixed account (please send the whole string of characters). For off campus users you can set up a PO or we can send the financial contact person a bill — your choice. Non-UW system users are charged an additional institutional overhead.

Adding a member to an existing lab:

    • Name of the PI whose lab you are joining.
    • Your name, UW NetID, phone number and email.

Sample Submission

Please contact nextgen-seq@biotech.wisc.edu to arrange all sample submissions. Samples and submission forms must be submitted before a project can be entered into our queue.

Submission Forms and Guidelines

Please check the service page guidelines for specific Submission Forms and Guidelines that need to be included with your samples.

Submitting Samples/ Shipping Instructions

After setting up a customer account, filling out submission form, and reviewing submission guidelines, you can now submit or send samples. *We require customers to submit electronic Submission Forms!*

In Person Submission

Please give advance notice of submission date and time so staff can be prepared to receive samples. Schedule a time for drop off with a staff member at nextgen-seq@biotech.wisc.edu

Shipping Submission

Please send the tracking information to:

Next Generation Sequencing: nextgen-seq@biotech.wisc.edu

*Samples that are being shipped must have a hard copy of the submission form and guidelines in the package! This way we know what the samples are and where they are from.*

If shipping samples from outside UWBC, ship via express shipping carrier on dry ice to the address below:

Next Generation Sequencing
UW DNA Sequencing Facility
Attn: Next Generation Sequencing
425 Henry Mall
1250 Biotechnology
Madison WI, 53706

High-Throughput Extraction Guidelines

Submission Forms and Guidelines

Submitting Samples

Please provide the guidelines and sample sheet form with sample names, status of the material (live, fixed, etc), biosafety level, and any other relevant safety information. Sample submission guidelines vary depending on the tissue type. Please read through and adhere to the specified instructions for your tissue type of interest.

Sample Types

  • Buccal Swabs

    – Very fresh, bristle-style swabs generally yield the best results

    -Swabs must be clearly labeled with sample name, date, and PI

  • Whole Blood (Low-throughput)

    -Up to 10 mL anticoagulated whole blood in shatterproof vials

    -Must be stored in either EDTA (purple top), Sodium Citrate (light blue), Acid Citrate Dextrose–ACD (yellow)

    -Vials must be clearly labeled with sample name, date, and PI

  • Whole Blood (High-throughput)

    -200 uL anticoagulated whole blood in shatterproof vials

    -Must be stored in either EDTA (purple top), Sodium Citrate (light blue), Acid Citrate Dextrose–ACD (yellow)

    -Vials must be clearly labeled with sample name, date, and PI

  • Mammalian Tissue

    -Up to 20 mg tissue in racked Qiagen Collection microtubes and caps (cat. #’s 19560 and 19566) UWBC can supply Qiagen Microtubes and caps upon request

    -Plates must be clearly labeled with plate name, date, and PI

    -Samples must be oriented in a column-based order (ie. A1, B1, C1….H1, A2, B2…H2)

  • Oragene Saliva Kits

    -Contents of clearly labeled Oragene collection vessel

    -Vessel must be clearly labeled with sample name, date, and PI

  • Fecal & Soil Samples

    -250 mg input material

    -Submit material in either PowerBead Pro tubes (UWBC can provide), collection and stabilization devices (DNA Genotek, Zymo, etc), or 1.5 mL/2 mL tubes (cryovials with external threads preferred) Please contact us via email about all other vessel formats

    -Sample vessels must be clearly labeled with sample name, date, and PI

  • Plant Tissue

    -Total of 1 cm2  leaf cutting (<50 mg), fresh or lyophilized, in racked Qiagen Collection Microtubes and caps ONLY (cat. #s 19560 and 19566) UWBC can supply Qiagen Microtubes and caps upon request

    -Plates must be clearly labeled with plate name, date, and PI

    -Samples must be oriented in a column-based order (ie A1, B1, C1….H1, A2, B2….H2)

  • Other

    For all other sample types please contact our extraction specialist at nextgen-seq@biotech.wisc.edu for more information

Extraction Policies

Policies

All human samples collected during and after November 2019 are considered COVID-19 pandemic specimens. If you would like DNA extracted from these types of samples please contact the facility to discuss the details of your project.

Samples that require handling beyond BSL2 containment, select agents, and biological toxins are NOT accepted. As a courtesy to our staff, inform us when samples are known to be pathogenic.

Unless other arrangements have been made, residual samples will not be returned. All residual samples will be autoclaved after DNA extraction and/or downstream testing is completed. It is the submitter’s responsibility to ensure UWBC’s disposal procedure is aligned with submitter’s biosafety protocol.

Extracted DNA can be returned to submitting lab once any downstream testing is completed. If submitting lab does not want the extracted DNA returned, it will be discarded after six months unless other arrangements have been made.

EXTERNAL RATES: Clients not affiliated with UW Madison or the University of Wisconsin system are charged a 55% surcharge for all labor and incidentals provided; prices are as listed above. This surcharge is subject to change.

Unless otherwise specified, all data and reagents distributed by the University of Wisconsin Biotechnology Center DNA Sequencing Facility are intended for research purposes only. They are not intended nor certified for diagnostic or clinical use. Clinical services are provided through our collaboration with the UW Collaborative Genomics Core.